6G7B
Nt2 domain of the TssA component from the type VI secretion system of Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.848, 101.316, 78.432 |
| Unit cell angles | 90.00, 104.63, 90.00 |
Refinement procedure
| Resolution | 101.320 - 1.760 |
| R-factor | 0.27382 |
| Rwork | 0.272 |
| R-free | 0.30575 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.702 |
| Data reduction software | xia2 |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.320 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.065 | |
| Rpim | 0.039 | |
| Number of reflections | 59629 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M Sodium chloride, 0.1 M HEPES buffer (pH 7.0), and 20% (w/v) PEG 6,000 |
