6G6F
Crystal structure of a parallel eight-helix coiled coil CC-Type2-LF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-07-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.000, 58.990, 47.050 |
| Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
| Resolution | 44.452 - 1.700 |
| R-factor | 0.1573 |
| Rwork | 0.156 |
| R-free | 0.18430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.749 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.990 | 58.990 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.028 | 0.021 | 0.079 |
| Rmeas | 0.031 | 0.023 | 0.093 |
| Rpim | 0.012 | 0.009 | 0.047 |
| Total number of observations | 125674 | ||
| Number of reflections | 22185 | 179 | 711 |
| <I/σ(I)> | 36.7 | ||
| Completeness [%] | 92.6 | 99.7 | 56.4 |
| Redundancy | 5.7 | 5.9 | 3.6 |
| CC(1/2) | 1.000 | 0.999 | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 50 mM Tris and 10 % w/v PEG 6000 |
