6G6F

Crystal structure of a parallel eight-helix coiled coil CC-Type2-LF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-07-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	42.000, 58.990, 47.050
Unit cell angles	90.00, 109.13, 90.00

Refinement procedure

Resolution	44.452 - 1.700
R-factor	0.1573
Rwork	0.156
R-free	0.18430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.749
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.990	58.990	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.028	0.021	0.079
Rmeas	0.031	0.023	0.093
Rpim	0.012	0.009	0.047
Total number of observations	125674
Number of reflections	22185	179	711
<I/σ(I)>	36.7
Completeness [%]	92.6	99.7	56.4
Redundancy	5.7	5.9	3.6
CC(1/2)	1.000	0.999	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	50 mM Tris and 10 % w/v PEG 6000