6G6C
Crystal structure of a parallel six-helix coiled coil CC-Type2-LL-L17E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-09-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.319, 117.940, 59.240 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 58.970 - 1.550 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.575 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 58.970 | 58.970 | 1.580 |
| High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
| Rmerge | 0.071 | 0.034 | 0.831 |
| Rmeas | 0.077 | 0.037 | 0.909 |
| Rpim | 0.031 | 0.014 | 0.362 |
| Total number of observations | 525877 | ||
| Number of reflections | 88233 | 562 | 4359 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6 | 6.4 | 6.1 |
| CC(1/2) | 0.999 | 0.999 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 50 mM SPG Buffer (Succinic acid, Sodium phosphate monobasic monohydrate and Glycine) and 12.5% w/v PEG 1500 |
