6G6B
Crystal structure of a parallel six-helix coiled coil CC-Type2-LL-L17Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-06-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 104.790, 104.790, 104.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.098 - 2.300 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.309 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.100 | 74.100 | 2.380 |
| High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
| Rmerge | 0.082 | 0.031 | 1.365 |
| Rmeas | 0.083 | 0.031 | 1.378 |
| Rpim | 0.010 | 0.004 | 0.182 |
| Total number of observations | 607009 | ||
| Number of reflections | 9275 | 216 | 897 |
| <I/σ(I)> | 38 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 65.4 | 50.8 | 56.1 |
| CC(1/2) | 1.000 | 1.000 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 50 mM SPG Buffer (Succinic acid, Sodium phosphate monobasic monohydrate and Glycine) and 12.5% w/v PEG 1500 |
