6G6A

Crystal structure of a parallel six-helix coiled coil CC-Type2-LL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-10-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 41 3 2
Unit cell lengths	103.529, 103.529, 103.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.206 - 2.701
R-factor	0.221
Rwork	0.218
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.598
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER (2.6.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.210	73.210	2.830
High resolution limit [Å]	2.700	8.960	2.700
Rmerge	0.209	0.052	1.849
Rmeas	0.211	0.052	1.863
Rpim	0.026	0.007	0.225
Total number of observations	365480
Number of reflections	5654	205	730
<I/σ(I)>	21.1
Completeness [%]	100.0	100	100
Redundancy	64.6	47.7	67.4
CC(1/2)	1.000	1.000	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	10 mM Sodium/Potassium phosphate, 50 mM Bis-Tris propane and 10 % w/v PEG 3350