6G5V
The X-ray structure of the adduct formed in the reaction between lysozyme and a platinum(II) terpyridine compound (pH 7.0)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.455, 79.455, 36.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.180 - 1.960 |
| R-factor | 0.19093 |
| Rwork | 0.188 |
| R-free | 0.24993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.180 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.084 | 0.478 |
| Rpim | 0.040 | 0.256 |
| Number of reflections | 8823 | |
| <I/σ(I)> | 8.1 | 3.1 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 2.8 | 2.4 |
| CC(1/2) | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.8 M succinic acid/NaOH, 0.10 M HEPES pH 7.0, 1% MPEG 2K |
