6G5E

MamM CTD H264A-E289A-C267S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.975
Spacegroup name	C 2 2 21
Unit cell lengths	37.027, 94.793, 53.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.480 - 1.600
R-factor	0.2087
Rwork	0.206
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5x
RMSD bond length	0.006
RMSD bond angle	0.805
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	1.620
High resolution limit [Å]	1.600	1.600
Rmerge	0.029
Rmeas	0.032
Rpim	0.013
Number of reflections	12814	553
<I/σ(I)>	26.9	2.2
Completeness [%]	99.2	86.5
Redundancy	6.4	5.8
CC(1/2)	0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Li2SO4, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350