6G5E
MamM CTD H264A-E289A-C267S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.027, 94.793, 53.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.480 - 1.600 |
| R-factor | 0.2087 |
| Rwork | 0.206 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.805 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.029 | |
| Rmeas | 0.032 | |
| Rpim | 0.013 | |
| Number of reflections | 12814 | 553 |
| <I/σ(I)> | 26.9 | 2.2 |
| Completeness [%] | 99.2 | 86.5 |
| Redundancy | 6.4 | 5.8 |
| CC(1/2) | 0.999 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Li2SO4, 0.1M BIS-TRIS pH=5.5, 25% PEG 3350 |
