6G57
Structure of the H1 domain of human KCTD8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 92.117, 92.117, 219.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.775 - 2.800 |
| R-factor | 0.2963 |
| Rwork | 0.295 |
| R-free | 0.34210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Human KCTD16 H1 (unpublished) |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.299 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.790 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 15121 | 1070 |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.5 | 18.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50% PEG200 -- 0.2M sodium chloride -- 0.1M sodium/potassium phosphate pH 7.5 |
