6G57

Structure of the H1 domain of human KCTD8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 61 2 2
Unit cell lengths	92.117, 92.117, 219.355
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	79.775 - 2.800
R-factor	0.2963
Rwork	0.295
R-free	0.34210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Human KCTD16 H1 (unpublished)
RMSD bond length	0.001
RMSD bond angle	0.299
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.790	2.820
High resolution limit [Å]	2.750	2.750
Number of reflections	15121	1070
<I/σ(I)>	16.1
Completeness [%]	100.0	100
Redundancy	18.5	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50% PEG200 -- 0.2M sodium chloride -- 0.1M sodium/potassium phosphate pH 7.5