6G4J
Structure of the protein kinase YabT from Bacillus subtilis in complex with an alphaREP crystallization helper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 67.690, 122.870, 50.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.513 - 1.599 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.21690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.599 | 1.599 |
| Rmeas | 0.053 | |
| Number of reflections | 56285 | 8958 |
| <I/σ(I)> | 14.11 | 1.28 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | PEG 8000, Hepes, |
