6G4F
Crystal structure of the omega TRANSAMINASE FROM PSEUDOMONAS Jessenii in complex with PMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 97.562, 97.562, 119.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.900 - 2.500 |
| R-factor | 0.16216 |
| Rwork | 0.160 |
| R-free | 0.20326 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | D_1200009303 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.900 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rpim | 0.062 | 0.420 |
| Number of reflections | 38677 | 4326 |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.0 M Sodium succinate pH 7.5, 0.1 mM PLP |
