6G4E
Crystal structure of the omega TRANSAMINASE FROM PSEUDOMONAS Jessenii in complex with PLP and 6-aminohexanoate (6-ACA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 98.000, 98.000, 119.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.100 - 2.450 |
| R-factor | 0.16666 |
| Rwork | 0.165 |
| R-free | 0.20437 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | D_1200009303 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.100 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rpim | 0.070 | 0.404 |
| Number of reflections | 41576 | 4646 |
| <I/σ(I)> | 7.9 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.0 M Sodium succinate pH 7.5, 0.1 mM PLP |
