6G44

Crystal structure of mavirus major capsid protein lacking the C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	131.190, 132.580, 135.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.330 - 1.500
R-factor	0.1658
Rwork	0.165
R-free	0.18290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g43
RMSD bond length	0.010
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.330	47.330	1.600
High resolution limit [Å]	1.500	20.000	1.500
Rmerge	0.077	0.064	0.410
Rmeas	0.085	0.073	0.452
Number of reflections	372727	183	65183
<I/σ(I)>	12.89	23.9	3.95
Completeness [%]	99.6	90.6	99.7
Redundancy	5.575	4.481	5.55
CC(1/2)	0.996	0.976	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1 M tri-sodium citrate, 1.2 - 1.7 M ammonium sulfate