6G42

Crystal structure of mavirus penton protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	95.240, 131.100, 154.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.860 - 2.700
R-factor	0.2229
Rwork	0.220
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g41
RMSD bond length	0.007
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.860	47.860	2.800
High resolution limit [Å]	2.700	10.000	2.700
Rmerge	0.085	0.037	0.721
Rmeas	0.093	0.041	0.783
Number of reflections	53606	1168	5446
<I/σ(I)>	16.22	40.11	3.08
Completeness [%]	99.8	98.5	99.6
Redundancy	6.68	5.871	6.672
CC(1/2)	0.997	0.996	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris, 0.3 M NaCl, 26% PEG3350