6G41
Crystal structure of SeMet-labeled mavirus penton protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.790, 94.330, 156.380 |
| Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
| Resolution | 49.090 - 2.900 |
| R-factor | 0.2171 |
| Rwork | 0.214 |
| R-free | 0.26950 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.090 | 49.090 | 3.000 |
| High resolution limit [Å] | 2.900 | 10.000 | 2.900 |
| Rmerge | 0.139 | 0.068 | 0.682 |
| Rmeas | 0.142 | 0.070 | 0.700 |
| Total number of observations | 3417378 | ||
| Number of reflections | 163213 | 3942 | 15717 |
| <I/σ(I)> | 19.04 | 40.93 | 5.49 |
| Completeness [%] | 99.3 | 98.6 | 99 |
| Redundancy | 20.938 | 19.831 | 19.991 |
| CC(1/2) | 0.999 | 0.999 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris, 0.37 M NaCl, 24% PEG3350 |
