6G3S

Structure of tellurium-centred Anderson-Evans polyoxotungstate (TEW) bound to the nucleotide binding domain of HSP70. Second structure of two TEW-HSP70 structures deposited.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	69.900, 70.967, 99.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.510 - 2.300
R-factor	0.21657
Rwork	0.215
R-free	0.24974
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hjo
RMSD bond length	0.015
RMSD bond angle	1.656
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.520	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.074	0.500
Rmeas	0.088	0.600
Number of reflections	22131	6702
<I/σ(I)>	10.98	1.87
Completeness [%]	97.0	97.8
Redundancy	3.37	3.33
CC(1/2)	0.998	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Bis-Tris pH 6.5, 0.2 M NaCl, 25% PEG 3350