6G3R

Structure of tellurium-centred Anderson-Evans polyoxotungstate (TEW) bound to the nucleotide binding domain of HSP70. Structure one of two TEW-HSP70 structures deposited.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-01
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	46.441, 64.691, 143.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.030 - 1.400
R-factor	0.14083
Rwork	0.139
R-free	0.17998
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.030
RMSD bond angle	2.376
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	1.490
High resolution limit [Å]	1.400	1.400
Rmerge		0.800
Number of reflections	151677
<I/σ(I)>	10.14	1.31
Completeness [%]	92.4	95.8
Redundancy	5
CC(1/2)		0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 8.5, 25% PEG 3350