6G30

Crystal structure of the p97 D2 domain in a helical split-washer conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 61
Unit cell lengths	94.761, 94.761, 66.368
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.380 - 2.418
R-factor	0.229
Rwork	0.227
R-free	0.27290
RMSD bond length	0.010
RMSD bond angle	1.389
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.380	2.504
High resolution limit [Å]	2.418	2.418
Number of reflections	13089
<I/σ(I)>	21.75
Completeness [%]	98.5
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.08M L-Na-Glutamate; 0.08M Alanine (racemic); 0.08M Glycine; 0.08M Lysine HCl (racemic); 0.08M Serine (racemic), 0.08M Tris, 0.08M BICINE, 10% v/v MPD; 10% PEG 1000; 10% w/v PEG 3350 at pH 8.5