6G2U

Crystal structure of the human glutamate dehydrogenase 2 (hGDH2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	121.795, 149.304, 433.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.651 - 2.934
R-factor	0.195747385819
Rwork	0.193
R-free	0.24881
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l1f
RMSD bond length	0.002
RMSD bond angle	0.387
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.150	3.090
High resolution limit [Å]	2.930	2.930
Rmeas	0.214
Number of reflections	83838	11344
<I/σ(I)>	13.9
Completeness [%]	98.9	92.6
Redundancy	13.6	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	PEG 8000, MPD, NaCl, sodium phosphate