6G2U
Crystal structure of the human glutamate dehydrogenase 2 (hGDH2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.795, 149.304, 433.315 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.651 - 2.934 |
| R-factor | 0.195747385819 |
| Rwork | 0.193 |
| R-free | 0.24881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l1f |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.387 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.150 | 3.090 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmeas | 0.214 | |
| Number of reflections | 83838 | 11344 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 98.9 | 92.6 |
| Redundancy | 13.6 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG 8000, MPD, NaCl, sodium phosphate |
