6G2O
X-ray structure of NSD3-PWWP1 in complex with compound BI-9321
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.279, 47.568, 61.113 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.710 - 1.810 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22400 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.870 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | BALBES |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.710 | 1.910 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.089 | |
Number of reflections | 12538 | 1756 |
<I/σ(I)> | 14 | 2.4 |
Completeness [%] | 92.8 | 100 |
Redundancy | 6.1 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 100mM Morpheus Buffer 3, 30% P550MME_P20K, 10% Morpheus Ethylene glycols |