6G2A
Human [protein ADP-ribosylargenine] hydrolase ARH1 in complex with ADP-HPM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0036 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.526, 42.940, 89.231 |
| Unit cell angles | 90.00, 118.93, 90.00 |
Refinement procedure
| Resolution | 78.090 - 1.800 |
| R-factor | 0.18351 |
| Rwork | 0.181 |
| R-free | 0.22039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hfw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.455 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.090 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.488 |
| Rmeas | 0.083 | 0.575 |
| Rpim | 0.044 | 0.302 |
| Number of reflections | 30388 | 1774 |
| <I/σ(I)> | 12.8 | 2.7 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 6.4 | 5 |
| CC(1/2) | 0.999 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 100 mM magnesium acetate, 12 % (w/v) PEG8000, 100 mM MOPS pH 7.5 |
