6G1Y
Crystal structure of the photosensory core module (PCM) of a bathy phytochrome from Agrobacterium fabrum in the Pfr state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.520, 93.360, 174.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.090 - 2.500 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c2w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.090 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.187 | |
| Number of reflections | 42806 | |
| <I/σ(I)> | 8.6 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.6 |
| CC(1/2) | 0.989 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | MPD, VAPOR DIFFUSION, TEMPERATURE 277K |
