6G1D
Corynebacterium glutamicum OxyR C206 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-02 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98015 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.409, 63.481, 157.414 |
| Unit cell angles | 90.00, 97.79, 90.00 |
Refinement procedure
| Resolution | 44.864 - 1.992 |
| R-factor | 0.2009 |
| Rwork | 0.200 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x6g |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.664 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.230 | 49.230 | 2.030 |
| High resolution limit [Å] | 1.990 | 10.910 | 1.990 |
| Rmerge | 0.340 | 0.201 | 1.882 |
| Rmeas | 0.367 | 0.217 | 2.046 |
| Rpim | 0.138 | 0.082 | 0.789 |
| Total number of observations | 692313 | ||
| Number of reflections | 98727 | 658 | 4132 |
| <I/σ(I)> | 2.8 | ||
| Completeness [%] | 99.0 | 99.1 | 85.1 |
| Redundancy | 7 | 6.7 | 6.1 |
| CC(1/2) | 0.958 | 0.944 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 283 | PEG 4000, 2-(N-morpholino)ethanesulfonic acid (MES), lithium sulfate, TCEP |
