6G13
C-terminal domain of MERS-CoV nucleocapsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.978714 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.680, 120.680, 92.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.330 - 1.970 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jw8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER (PHENIX 1.10.1-2155) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 55287 | |
| <I/σ(I)> | 16.12 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium nitrate, 2.2M AmSO4 |
