6G12

Crystal structure of GMPPNP bound RbgA from S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97903
Spacegroup name	P 21 21 21
Unit cell lengths	71.899, 77.710, 124.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.245 - 1.929
R-factor	0.2283
Rwork	0.227
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6g0z
RMSD bond length	0.008
RMSD bond angle	1.119
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.245	2.000
High resolution limit [Å]	1.929	1.930
Number of reflections	99071
<I/σ(I)>	6.36
Completeness [%]	99.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Potassium fluoride, 0.1 M MES pH 6.0, 20% PEG 3350