6G0V

Human Galectin-3 in complex with a TF tumor-associated antigen mimetic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	35.900, 58.200, 62.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.675 - 1.090
R-factor	0.1541
Rwork	0.153
R-free	0.17710
RMSD bond length	0.007
RMSD bond angle	1.035
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.675	1.140
High resolution limit [Å]	1.090	1.090
Rmerge	0.040
Rpim	0.019
Number of reflections	41602
<I/σ(I)>	17.8
Completeness [%]	76.2
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	34% (w/v) PEG 400, 100 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 8 mM B-mercaptoethanol