6G00
Crystal Structure of a GH8 xylanase from Teredinibacter turnerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.540, 72.995, 90.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.970 - 1.400 |
| R-factor | 0.15369 |
| Rwork | 0.153 |
| R-free | 0.17142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wu4 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.683 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 80610 | |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.4 | |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291.2 | sodium acetate (0.1M, pH 5.0), NaCl (0.2 M), polyethylene glycol 6000 16%. |
