6FZE
BBE31 from Lyme disease agent Borrelia (Borreliella) burgdorferi playing a vital role in successful colonization of the mammalian host (native data)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.820, 77.410, 87.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.950 - 2.260 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fxe |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.833 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.950 | 2.390 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 23985 | 3442 |
| <I/σ(I)> | 11.9 | 3.9 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 0.2 M Ammonium sulfate 0.1 M Sodium acetate 24% PEG 2000 |
