6FZC
Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant L184F/L360F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-09 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.140, 71.193, 113.946 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.483 - 2.700 |
R-factor | 0.2226 |
Rwork | 0.222 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x6u |
RMSD bond length | 0.026 |
RMSD bond angle | 1.704 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.890 | 2.603 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.039 | |
Rmeas | 0.042 | |
Number of reflections | 15480 | |
<I/σ(I)> | 45.2 | |
Completeness [%] | 99.0 | |
Redundancy | 7.7 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M sodium citrate, 25% PEG 3350 |