6FZ7

Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07227
Spacegroup name	P 21 21 21
Unit cell lengths	49.970, 71.680, 114.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.255 - 1.736
R-factor	0.1714
Rwork	0.171
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x6u
RMSD bond length	0.025
RMSD bond angle	1.555
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.255	1.736
High resolution limit [Å]	1.736	1.736
Rmerge	0.080
Rmeas	0.090
Number of reflections	43045
<I/σ(I)>	11.8
Completeness [%]	99.4
Redundancy	5.4
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium citrate, 25% PEG 3350