6FZ1

Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L360F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	273
Detector technology	PIXEL
Collection date	2017-05-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07227
Spacegroup name	P 21 21 21
Unit cell lengths	49.950, 71.510, 113.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.527 - 2.200
R-factor	0.2068
Rwork	0.206
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4x6u
RMSD bond length	0.025
RMSD bond angle	1.669
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.510	71.510
High resolution limit [Å]	2.200	2.200
Rmerge	0.077
Rmeas	0.083
Number of reflections	21096
<I/σ(I)>	20
Completeness [%]	98.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M sodium formate, 20% PEG 3350