6FYQ
The crystal structure of a new transaminase from the marine bacterium Virgibacillus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.886, 100.886, 97.411 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.860 - 2.000 |
| R-factor | 0.1792 |
| Rwork | 0.177 |
| R-free | 0.21920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tib |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.393 |
| Rmeas | 0.046 | 0.454 |
| Rpim | 0.023 | 0.223 |
| Number of reflections | 38942 | 5629 |
| <I/σ(I)> | 17.3 | 3.2 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.999 | 0.888 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Morpheus condition D10 (0.12 M Alcohols, 0.1 M Buffer System 3 pH 8.5, 50% (v/v) Precipitant Mix 2 (Molecular dimensions) |
