6FYG
The crystal structure of EncM V135T mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99997 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 164.806, 174.229, 131.773 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.686 - 2.550 |
R-factor | 0.2029 |
Rwork | 0.201 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xlo |
RMSD bond length | 0.005 |
RMSD bond angle | 0.795 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.690 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.130 | 0.540 |
Number of reflections | 61948 | |
<I/σ(I)> | 18.1 | |
Completeness [%] | 100.0 | |
Redundancy | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M HEPES-Na, 0.2M Calcium Acetate, 20% - 30% PEG 3350, pH 7.5 |