6FYD

The crystal structure of EncM T139V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99987
Spacegroup name	C 2 2 2
Unit cell lengths	161.742, 175.264, 129.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.880 - 1.760
R-factor	0.2059
Rwork	0.204
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xlo
RMSD bond length	0.011
RMSD bond angle	1.106
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.880	1.850
High resolution limit [Å]	1.760	1.760
Rmerge	0.070	0.540
Number of reflections	180057
<I/σ(I)>	17.5
Completeness [%]	99.5
Redundancy	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M HEPES-Na, 0.2M Calcium Acetate, 20% - 30% PEG 3350, pH 7.5