6FYA

The crystal structure of EncM under anaerobic conditions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	79.260, 85.399, 126.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.399 - 2.600
R-factor	0.2317
Rwork	0.229
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xlo
RMSD bond length	0.004
RMSD bond angle	0.772
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11rc1_2513: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	126.620	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.180	0.460
Number of reflections	26304
<I/σ(I)>	4.8
Completeness [%]	97.5
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M HEPES-Na, 0.2M Calcium Acetate, 20-30% PEG 3350, pH 7.5