6FXP

Crystal structure of S. aureus glucosaminidase B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.894290
Spacegroup name	P 61 2 2
Unit cell lengths	151.770, 151.770, 122.510
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.950 - 2.030
R-factor	0.212
Rwork	0.212
R-free	0.21970
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	MAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.660	2.150
High resolution limit [Å]	2.030	2.030
Rmeas	0.091
Number of reflections	53891
<I/σ(I)>	27
Completeness [%]	99.8
Redundancy	18.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.75	293.15	0.1 M Na3 citrate, 45 % PEG 200