6FXM

Crystal Structure of full-length Human Lysyl Hydroxylase LH3 - Cocrystal with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-11
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 2 2 21
Unit cell lengths	97.270, 99.949, 225.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.370 - 2.100
R-factor	0.2299
Rwork	0.229
R-free	0.25180
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.618
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.790	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.095	2.444
Rpim	0.054	1.389
Number of reflections	64193	4455
<I/σ(I)>	12.2	0.9
Completeness [%]	99.8	99.8
Redundancy	7.8	8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH, 0.5 mM MnCl2