6FXK
Crystal Structure of full-length Human Lysyl Hydroxylase LH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-02 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.290, 100.140, 226.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.780 - 2.700 |
| R-factor | 0.2795 |
| Rwork | 0.278 |
| R-free | 0.30870 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.463 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.780 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.166 | 1.309 |
| Rpim | 0.146 | 1.163 |
| Number of reflections | 30019 | 3967 |
| <I/σ(I)> | 4.6 | 0.7 |
| Completeness [%] | 98.0 | 98.5 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.990 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH |
