6FXK

Crystal Structure of full-length Human Lysyl Hydroxylase LH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-11-02
Detector	DECTRIS PILATUS 12M
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	97.290, 100.140, 226.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.780 - 2.700
R-factor	0.2795
Rwork	0.278
R-free	0.30870
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.780	2.830
High resolution limit [Å]	2.700	2.700
Rmerge	0.166	1.309
Rpim	0.146	1.163
Number of reflections	30019	3967
<I/σ(I)>	4.6	0.7
Completeness [%]	98.0	98.5
Redundancy	3.3	3.3
CC(1/2)	0.990	0.435

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	277	600 mM sodium formate, 12% PGA-LM, 100 mM HEPES/NaOH