6FXJ
Structure of coproheme decarboxylase from Listeria monocytogenes in complex with iron coproporphyrin III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.870, 129.110, 77.920 |
| Unit cell angles | 90.00, 105.96, 90.00 |
Refinement procedure
| Resolution | 48.890 - 1.790 |
| R-factor | 0.186861158096 |
| Rwork | 0.186 |
| R-free | 0.21368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5loq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.669 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2719) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.134 | 1.147 |
| Rmeas | 0.159 | 1.351 |
| Rpim | 0.083 | 0.708 |
| Number of reflections | 134739 | |
| <I/σ(I)> | 6.98 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1 M HEPES pH 7.5 20 %(w/v) PEG 4000 10 % (v/v) 2-propanol |
