6FXA
Dimerization domain of TP901-1 CI repressor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.840, 42.560, 101.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.225 - 1.500 |
R-factor | 0.1481 |
Rwork | 0.145 |
R-free | 0.20010 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.225 | 40.225 | 1.540 |
High resolution limit [Å] | 1.500 | 6.700 | 1.500 |
Rmerge | 0.175 | 0.042 | 0.791 |
Rmeas | 0.183 | 0.044 | 0.828 |
Number of reflections | 31189 | 430 | 2226 |
<I/σ(I)> | 15.22 | 55.09 | 2.74 |
Completeness [%] | 99.9 | 99.5 | 98.7 |
Redundancy | 11.95 | 10.009 | 11.289 |
CC(1/2) | 0.998 | 1.000 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 0.2 M lithium sulfate, 0.1 M sodium acetate |