6FX8
The X-ray structure of the ferritin nanocage containing Au and Pt, obtained upon encapsulation of a single heterobimetallic compound within the protein cage (rotating anode data)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-08 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.832, 180.832, 180.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.400 - 1.800 |
| R-factor | 0.16542 |
| Rwork | 0.164 |
| R-free | 0.19745 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erk |
| RMSD bond length | 0.044 |
| RMSD bond angle | 2.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.400 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.104 | 0.717 |
| Rpim | 0.052 | 0.402 |
| Number of reflections | 24060 | |
| <I/σ(I)> | 18.4 | 2 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 8.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.6 M ammonium sulfate 0.1 M Tris HCl pH 7.4 60 mM cadmium sulfate |
