6FX5
MITF dimerization mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.720, 92.830, 86.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.045 - 2.050 |
| R-factor | 0.2258 |
| Rwork | 0.224 |
| R-free | 0.24630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ath |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.385 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.045 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 9762 | 987 |
| <I/σ(I)> | 7.8 | 3.5 |
| Completeness [%] | 96.5 | 98 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.990 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | Crystals grew in conditions between 2.0 M and 2.4 M Ammonium sulfate and 0.1 and 0.15 M Cadmium Sulfate |
