6FWZ

Crystal structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) (V264G mutant) in complex with UDP-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 65 2 2
Unit cell lengths	102.459, 102.459, 238.209
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.100
R-factor	0.22327
Rwork	0.223
R-free	0.23624
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lev
RMSD bond length	0.012
RMSD bond angle	1.554
Data reduction software	XDS (October 15, 2015)
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.550	3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.095	2.376
Rmeas	0.101	2.505
Rpim	0.033	0.784
Number of reflections	14197	1002
<I/σ(I)>	14.4	1.1
Completeness [%]	100.0	100
Redundancy	9.5	9.9
CC(1/2)	0.999	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1M bicine pH 9.0 -- 0.05M sodium chloride -- 37.5% PEG300