6FWR

Structure of DinG in complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97951
Spacegroup name	P 21 21 2
Unit cell lengths	98.971, 133.867, 58.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.862 - 2.500
R-factor	0.2125
Rwork	0.211
R-free	0.24570
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.134
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.590	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	27857
<I/σ(I)>	12.3
Completeness [%]	100.0	98
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.25 M KCl, 0.1M HEPES (pH 7.8) and 22% PEG 3350.