6FWB
Crystal structure of Mat2A at 1.79 Angstron resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.07822 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.430, 103.027, 114.465 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 57.130 - 1.790 |
| R-factor | 0.17244 |
| Rwork | 0.172 |
| R-free | 0.18317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.556 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.130 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.887 | |
| Rmeas | 0.961 | |
| Rpim | 0.365 | |
| Number of reflections | 147112 | 10821 |
| <I/σ(I)> | 12.7 | 2.2 |
| Completeness [%] | 98.8 | 98.7 |
| Redundancy | 7.1 | 6.8 |
| CC(1/2) | 0.737 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 293 | 35% PEG 1000, 0.1M Hepes, 50mM Li2SO4 |
