6FVQ
The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - R86A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2017-12-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 216.237, 112.475, 144.461 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.205 |
| Rwork | 0.204 |
| R-free | 0.21700 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 47694 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 150mM MgCl2 35%PEG200 Tris pH 8.0 3% DMSO |
