6FV5

QTRT2, the non-catalytic subunit of murine tRNA-Guanine Transglycosylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918400
Spacegroup name	P 21 21 2
Unit cell lengths	118.475, 124.573, 61.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.781 - 2.179
R-factor	0.1711
Rwork	0.170
R-free	0.20750
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	HKL2Map
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.430	2.310
High resolution limit [Å]	2.179	2.300	2.179
Number of reflections	46914	12669	6374
<I/σ(I)>	15.56	4.38	2.7
Completeness [%]	96.4	99	82.2
Redundancy	6.5	13.5	5.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.5	100 mmol/l Bis Tris propane pH 6.5 200 mmol/l Sodium malonate 25 % PEG 3350 10 mmol/l Betaine hydrochloride
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.5	100 mmol/l Bis Tris propane pH 6.5 200 mmol/l Sodium malonate 25 % PEG 3350 10 mmol/l Betaine hydrochloride