6FV0
Crystal structure of the TPR domain of KLC1 in complex with the C-terminal peptide of torsinA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.040, 89.680, 50.990 |
| Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
| Resolution | 52.500 - 2.290 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fuz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.080 | 0.832 |
| Rpim | 0.040 | 0.520 |
| Number of reflections | 21275 | 1021 |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 99.6 | 93.9 |
| Redundancy | 4.7 | 3.2 |
| CC(1/2) | 0.997 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.1 M Hepes pH 7.5, 30% propan-2-ol, 20% PEG4000 |
