6FUE
Periplasmic coiled coil domain of the FapF amyloid transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.590, 37.700, 57.340 |
| Unit cell angles | 90.24, 108.95, 119.89 |
Refinement procedure
| Resolution | 32.010 - 1.780 |
| R-factor | 0.20923 |
| Rwork | 0.207 |
| R-free | 0.24797 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.856 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.010 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.030 | 0.309 |
| Rmeas | 0.420 | |
| Rpim | 0.027 | 0.282 |
| Number of reflections | 22562 | 2263 |
| <I/σ(I)> | 13.86 | 2.4 |
| Completeness [%] | 93.5 | 93.5 |
| Redundancy | 1.99 | 2 |
| CC(1/2) | 0.998 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Zinc acetate dihydrate, 0.1 M Sodium cacodylate, pH 6.5, 10 % v/v 2-Propanol |
