6FUE

Periplasmic coiled coil domain of the FapF amyloid transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	37.590, 37.700, 57.340
Unit cell angles	90.24, 108.95, 119.89

Refinement procedure

Resolution	32.010 - 1.780
R-factor	0.20923
Rwork	0.207
R-free	0.24797
Structure solution method	AB INITIO PHASING
RMSD bond length	0.019
RMSD bond angle	1.856
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.010	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.030	0.309
Rmeas		0.420
Rpim	0.027	0.282
Number of reflections	22562	2263
<I/σ(I)>	13.86	2.4
Completeness [%]	93.5	93.5
Redundancy	1.99	2
CC(1/2)	0.998	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M Zinc acetate dihydrate, 0.1 M Sodium cacodylate, pH 6.5, 10 % v/v 2-Propanol