6FUD
Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikA with the HMA domain of Pikm-1 from rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.070, 54.440, 46.640 |
| Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
| Resolution | 34.760 - 1.300 |
| R-factor | 0.12719 |
| Rwork | 0.125 |
| R-free | 0.16191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a6w |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.775 |
| Data reduction software | xia2 (0.4.0.0) |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.760 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.043 | |
| Number of reflections | 33854 | |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.12M Alcohols (0.2M 1,6-Hexanediol; 0.2M 1-Butanol; 0.2M 1,2-Propanediol; 0.2M 2-Propanol; 0.2M 1,4-Butanediol; 0.2M 1,3-Propanediol); 0.1M Buffer system 2 (1M sodium HEPES, MOPS (acid)) pH 7.5; 50% v/v Precipitant mix 4 (25%v/v MPD; 25%v/v PEG 1000; 25%v/v PEG3350) |
