6FTO

Crystal structure of the Chp2 chromoshadow domain in complex with N-terminal domain of chromatin remodeler Mit1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99999
Spacegroup name	P 31 2 1
Unit cell lengths	58.053, 58.053, 139.492
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.297 - 1.600
R-factor	0.1721
Rwork	0.171
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e0b
RMSD bond length	0.010
RMSD bond angle	0.991
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.111	0.810
Rmeas	0.116	0.837
Rpim	0.037	0.296
Number of reflections	36873	1830
<I/σ(I)>	16.8	3.5
Completeness [%]	100.0	99.9
Redundancy	18.1	16.2
CC(1/2)	0.997	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	2.4 M sodium malonate, pH 7, 3% 1,6-hexanediol